ANIMAL DISEASE DEFENSE

 

 

 

Research Areas


Biological Systems

Information Analysis Systems

Education and Outreach


About Us

Institute for Infectious Animal Diseases (IIAD)
Texas A&M University
1500 Research Parkway, Suite B270
College Station, TX  77843
Tel:  979-845-2855
Email:  IIAD@ag.tamu.edu 
Website:  http://iiad.tamu.edu

Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD)
Kansas State University
1800 Denison Ave.
Mosier Hall K224
Manhattan, KS  66506-5606
Tel:  785-532-2793
Email:  jricht@vet.k-state.edu   Website:  http://www.ceezad.org

ZADD Factsheet

Leadership:

  • Institute for Infectious Animal Diseases: Director Gerald Parker, DVM, Ph.D., M.S.
  • CEEZAD: Director Jürgen A. Richt, DVM, Ph.D.

 

Project Search

Zoonotic and Animal Disease Defense

The Center of Excellence for Zoonotic and Animal Disease Defense (ZADD) protects the nation's agriculture and public health sectors against high-consequence transboundary, emerging, and zoonotic disease threats. The Center meets this mission by advancing the development of: * Next-generation vaccine cadidates, vaccine platforms and adjuvants against high-consequence animal diseases. * Decision support systems and emergency management tools to support prevention and control of and recovery from high-consequence animal and zoonotic diseases. * Integrated educational and training programs for the next generation of professional to work in disciplines that research, support and respond to transboundary, emerging, and zoonotic diseases.

Center Activity

Project Spotlights

IIAD: IIAD releases mobile app to enhance CVI submissions from the field

The Institute for Infectious Animal Diseases (IIAD), a Department of Homeland Security (DHS) Science and Technology Center of Excellence, in partnership with the Texas Center for Applied Technology (TCAT), a part of the Texas A&M Engineering Experiment Station, have developed a mobile Certificate of Veterinary Inspection (CVI) application to support veterinary practitioners submitting animal health certificate records from the field. The technology was developed as part of the Institute’s DHS funded business continuity project, and in close coordination and collaboration with state animal health officials (SAHOs) in Colorado and Kansas. The mobile application was modeled after and builds upon the eCVI PDF form developed by the SAHOs in these states.

The “iCVI” iPad app is currently available for free download from Apple’s App Store and provides an easy-to-use, touch-screen interface for digitally entering animal health certificate data. IIAD’s iCVI strives to expand the toolbox of capabilities available to veterinary practitioners, allowing them to easily submit electronic animal health certificates, or store that information within the application for forwarding when data connectivity becomes available. This real-time information sharing is an alternative to email or web-based systems, and helps improve communication between veterinarians and state animal health offices by supporting certificate submission from the field.

In addition to providing a mobile interface for CVIs, the end-user has the ability to print paper-based forms directly from the mobile application. Submitted CVIs can also be automatically and/or manually imported into state animal health information systems.

“This new technology will help streamline the work flow for our veterinarians in the field,” said Bill Brown, DVM, Kansas animal health commissioner. “We want to make sure that tools are available to improve efficiency, as well as provide connectivity to the animal health network.”

The submitted CVIs can also be made available within IIAD’s AgConnect suite. AgConnect is a suite of customizable data integration and analysis products designed to enhance real-time animal health situational awareness, enable permissioned data sharing and support decision-making in the event of emerging, zoonotic and/or high consequence diseases. With permissions, state veterinarians will be able to visualize the iCVI data along with other data stored within the AgConnect suite (e.g., additional animal movements, premises, surveillance, diagnostic test results and other emergency response data), allowing for greater situational awareness during a disease event. Real-time data integration of the above mentioned data streams would greatly aid state animal health officials in making decisions on animal and animal product movements during a disease outbreak.

“The iCVI has tremendous potential to provide real-time information on livestock movements and improve the accuracy of that information to animal health officials,” said Keith Roehr, DVM, state veterinarian of Colorado. “It will definitely enhance animal disease traceability, which ultimately safeguards animal and human health.”

iCVI is currently being piloted in eight states with several additional states looking to start their own pilot programs. During the pilot, IIAD will work closely with SAHOs and practicing veterinarians to refine and optimize this newly developed mobile application as needed. As part of future efforts, IIAD intends to expand iCVI to operate on other mobile platforms.

The app was also recently presented on a call to the National Assembly of State Animal Health Officials (NASAHO).

“There has been a significant interest from other state veterinarians in the iCVI project,” said Dr. Roehr, who is also a past president of the NASAHO. “This new development may be the ‘tipping point’ in moving veterinarians forward in using electronic means to issue certificates of veterinary inspection.”

To learn more about IIAD, visit http://iiad.tamu.edu.


IIAD: Introducing the Institute for Infectious Animal Diseases

Effective May 1, 2014, the National Center for Foreign Animal and Zoonotic Disease Defense (FAZD Center) has become the Institute for Infectious Animal Diseases (IIAD). While our name is changing, our mission—to perform research and develop products to defend the nation from high-consequence emerging and zoonotic diseases—remains the same.

Over the past several years, the FAZD Center has enjoyed success with new partners and projects across the U.S. and around the globe. As our work begins to encompass a broader audience in the animal health realm, we’ve taken a close look at how we present our work and ourselves to these audiences. In an effort to clarify our expanded, multidisciplinary mission and allow our national and global partners to have a greater understanding of our scope and capacity, the FAZD Center is getting a new name.

“This is an important day in the history of the Institute,” saidTammy R. Beckham, DVM, PhD, and Institute director. “The new name signals our continued commitment to serving the animal and public health sectors while working alongside our valued partners in government, academia and industry both domestically and globally.”

The Institute will continue to focus on developing solutions for transboundary, emerging and zoonotic animal diseases. The Institute remains a Department of Homeland Security Science and Technology Center of Excellence and will continue to leverage the resources of our international, national, state and local partners.

For more information and updated contact information, visit iiad.tamu.edu. Follow us on Twitter at @IIADnews for the latest updates on the Institute’s work.


IIAD: IIAD receives $2M in federal funds for new EPS technology

COLLEGE STATION, Texas – John Sharp, chancellor of The Texas A&M University System, today announced that the Institute for Infectious Animal Diseases (IIAD) received $2 million in federal funds from the Department of Homeland Security’s Science and Technology Directorate to develop a disease-surveillance technology designed to protect U.S. animal agriculture from potentially catastrophic outbreaks of infectious pathogens. The project has the potential for a total $9 million investment over a three-year period.

To read the entire press release, please visit the following URL: http://fazd.tamu.edu/2013/09/4997/


 

News & Press Release

 

November 7, 2014

 

JOINT NATIONAL PORK BOARD (NPB) AND CEEZAD REQUEST
FOR WHITE PAPERS.
CEEZAD is teaming up with the NPB for additional request for proposals.

The goal of this joint call is to leverage the research dollars that each organization has available for transboundary animal diseases (TAD) of swine while limiting duplication of efforts and to further the organizations' missions to prevent, detect and control these diseases. Priority diseases for both organizations include Foot-and-Mouth Disease, Classical Swine Fever and African Swine Fever.

Due date for this call for white paper submissions is December 8, 2014.

Full details here: http://www.pork.org/pork-checkoff-research/research-request-proposal/


November 7, 2014
CEEZAD Director receives Prestigious KSU Iman Outstanding Faculty Award for Research

Introduced in 2007, the annual Dr. Ron and Rae Iman Outstanding Faculty Awards are sponsored by the K-State Alumni Association and are made possible through the generosity of Ron and Rae Iman.

This year this most prestigious KSU award for research was given to Juergen A. Richt, Regents distinguished professor in the College of Veterinary Medicine and director of CEEZAD. The award recognizes faculty members who have distinguished themselves in their chosen profession and who have contributed significantly through research to improve the betterment of the educational experience, or whose research has had a significant impact on their area of study.

Read More at K-State Today


August 21, 2014
New Associate Director at CEEZAD

The US Department of Homeland Security's Center of Excellence for Emerging and Zoonotic Diseases (CEEZAD), headquartered at Kansas State University, has appointed Steven Ellsworth as its Associate Director. Dr. Ellsworth earned undergraduate and graduate degrees at South Dakota State University and his DVM degree at the University of Missouri. He brings thirty years of industrial veterinary medicine experience to CEEZAD. Twenty years of that experience involved directing biologicals research and development programs, and then as Vice President of Operations, for two firms that are now part of Merck Animal Health. During this time he oversaw government approval of dozens of vaccines, and managed lab and animal facilities, vaccine production operations, and associated budgets. For the past five years he has served as a consultant on a variety of technical, business, and regulatory issues with animal health firms and universities.

Dr. Ellsworth, commented: "I am excited to be a part of the CEEZAD program and to help it meet its mission. It will be a good personal challenge to apply my experience in a new setting."

Dr. Juergen Richt, Director of CEEZAD, commented: "I expect Dr. Ellsworth's broad industrial experience will be immensely helpful in many facets of the CEEZAD mission, including assistance with the preparation of proposals and grant applications and helping to arrange collaborations with various stakeholders and institutions. His business perspective will be a great asset to improve the accountability of many scientific projects and to ensure the early implementation of potential applications with commercial partners."

For further information, contact ceezad@ksu.edu or 785-532-2793.


Visit to CEEZAD by Air Force Academy Scholars

The Department of Homeland Security (DHS) Summer Research Program for Federal Service Academies included a month-long visit to a CEEZAD laboratory by a lieutenant colonel and a cadet from the US Air Force Academy. Lt. Col Craig Narasaki and Cadet John Rosenberg studied vaccine and diagnostic research programs at CEEZAD in the lab of Dr. Juergen Richt. Prior to the start of their work, the visitors from USAFA completed all the necessary safety and compliance training procedures. Also, their work in Dr. Richt's lab was approved by the KSU's Institutional Biosafety Committee (IBC). The aim of the DHS Program is to provide faculty and students from military academies a chance to build research collaborations. The visit has laid the foundation for future collaborations between CEEZAD and the US Air Force Academy's Department of Biology.

Watch this three-minute video for further details about an unusual and exciting academic collaboration: K-State Lifeline

 

Current Projects 2013/2014
IIAD Projects:

Engage to Excel (E2E) - Maintaining Business Continuity and Performing Risk Management during an Animal Disease Outbreak

PIs: Jim Wall, Keith Biggers, Jim Roth, Nick Striegel, and Marianne Ash, Texas Center for Applied Technology, Iowa State University, Colorado Department of Agriculture, and Indiana Board of Animal Health

Researchers from the Institute for Infectious Animal Diseases (IIAD) have been heavily engaged in the use of information technology and the implementation of decision support tools in each of these stakeholder communities for some time. These investigators are suitably positioned to address the requirements for exchange of information/ data across each of the communities from both a domain and technical perspective, especially focusing on business continuity planning and operations. This understanding is critical in the creation of risk management tools supporting the decision-making process. The connections between the IIAD and state and federal emergency responders provide the required interface with the respective stakeholder communities to promote integrated incident command. The E2E project applies proven technology developed for the U.S. Department of Homeland Security and the U.S. Department of Agriculture, and engages a rich network of stakeholder relationships in government, industry and academia.

RNA Particle Vaccines as a Platform for Foot and Mouth Disease Outbreak Preparedness

PI: Mark Mogler, HarrisVaccines

Alphavirus-derived replicon RNA particles (RP) have recently been developed as a veterinary vaccine platform.  This platform has demonstrated efficacy in several veterinary species, especially swine, against a range of pathogens.  It does not suffer from anti-vector immune interference and can be used repeatedly in the same animal without a decrease in efficacy. The production of RP vaccines does not involve the cultivation of pathogens and thus avoids the chief impediment to foot and mouth disease (FMD) vaccine production.

The major products of this project will be a panel of novel FMD RP vaccines that target a broad range of current strains from each of the seven serotypes.  Possible approval of the vaccines as licensed veterinary biological products may greatly aid their distribution and use in the future. Continued work to streamline and optimize the manufacturing conditions (e.g., cell concentration, media formulation, incubation periods, harvest protocols) for lead candidates based on new serotypes/topotypes will help move this technology down the TRL pathway as well as improve readiness to scale up in case of emergency.

Development of a Phylogenetic Tool for Integration with the AgConnect Suite of Tools in Support of Genomic Analysis, Surveillance, and Business Continuity

PIs: Tammy Beckham, Melissa Berquist, and Keith Biggers, IIAD and Texas Center for Applied Technology 

Phylogenetics (or comparative genomics), a sub-field of genomics, studies the evolutionary relationships between groups of organisms that are discovered through molecular sequencing data and morphological data matrices.  A properly developed phylogenetics capability, embedded in the AgConnect suite of tools, will enhance the capabilities of the suite and provide the capacity to better understand pathogen movements/emergence in our livestock populations in the U.S. With this capacity built into the suite, the end-user (state animal health official or epidemiologist) would have the ability to investigate strain movement and divergence across both time and space, as well as track and compare disease history and spread within individual production systems and across industries. In addition, this capability can support informed selection of vaccines and therapeutics within a region or individual production system. During times of high consequence disease outbreaks and the emergence of new pathogens this capability would be critical to monitoring strain divergence and selecting appropriate vaccines for use.

Universal Sample Preparation Tool for the Concentration, Enrichment, and Detection of Rift Valley Fever Viral Proteins

PIs: Ben Lepene and Kylene Kehn-Hall, Ceres Nanosciences and George Mason University

This project adapts a nanoparticle bait chemistry platform, Nanotrap, for use as a method for concentration and analysis of Rift Valley Fever viral (RVFv) proteins from serum and tissue culture homogenates. This technology will assist with further development of research and diagnostic assays for RVFv and could potentially be incorporated as a first step in sample preparation.

This novel sample preparation technology enables the capture of RVFv and increases the sensitivity of detection >100 fold over current detection systems. It is a simple, flexible and rapid approach with the potential to be incorporated in to current diagnostic workflows to provide increased sensitivity and safety, allowing increased speed and accuracy of diagnosis. While currently focused on RVFv, this agent-agnostic technology could be expanded to other pathogens or developed in to a multiplex system for concentration/detection of several pathogens for different downstream endpoints (both molecular and serological assays). In addition, a novel aspect of the Nanotrap particles is that they allow for ambient temperature preservation of captured analytes preventing the need for cold chain sample storage prior to analysis.

Continuation of the Career Development Program from the National Center for Foreign Animal and Zoonotic Disease Defense

PI: Heather Simmons, IIAD   

Since 2008, the goal of the IIAD Career Development Program has been to provide a graduate-level fellowship program that promotes workforce development into public practice or academia. Emphasis is placed on building future workforce capacity in HS-STEM fields related to transboundary, emerging and zoonotic disease defense.

The Matrix-Chaperone: Ambient Temperature Biospecimen Collection, Transport, and Banking, for Simplified Animal Disease Screening (Phase II)

PIs: Pam Ferro and Mike Hogan, Texas A&M Veterinary Medical Diagnostic Laboratory and IntegenX

This project is focused on development and testing of novel technology for the collection, preservation, and transport of biological samples at ambient temperature. The first phase of the project focused on lead compound identification for inactivating experimental samples in the “Matrix Chaperone” format. In phase II, the project is expanding to fine-tune and validate these tools using avian samples collected in the field using the sample collection devices developed during phase I. Validation is performed via collection and analysis of avian influenza virus (AIV) and Newcastle disease virus (NDV) from tracheal and cloacal swabs and avian blood draws, and analyzed via standard rt-PCR, virus isolation, and immunoassay methods, comparing samples placed in standard transport media to samples collected via Matrix Chaperone elastomers and swabs.

Workshops to Coordinate and Enhance the Agro-Security Enterprise: Phase II

PI: Tammy Beckham, IIAD

New tools and technologies are needed to protect the nation’s agriculture and public health sectors against high-consequence transboundary animal, emerging and zoonotic diseases. Systems are needed for rapid detection of, response to, and recovery from animal disease events to support business continuity and ensure livestock welfare. Identified needs include development of veterinary medical countermeasures, pen-side tests, hand held detection systems and in-process monitoring devices.  IIAD has been coordinating a series of workshops to coordinate and enhance the agro-security enterprise by pulling together subject matter experts and stakeholders to identifying gaps in research and technology.  One outcome of these workshops is the IIAD Agricultural Screening Tools portfolio which aims to produce tools that screen for high-consequence animal diseases while fitting into established animal agriculture business routines. 

5th International Symposium on Managing Animal Mortality, Products, By-Products, and Associated Health Risk: Connecting Research, Regulation, and Response

PIs: Heather Simmons and Dale Rozeboom, IIAD and Michigan State University

IIAD is coordinating an international symposium involving academic, government, and industry entities designed to stimulate discussion about research concerns and to identify gaps in response to disease outbreaks and emergency events involving livestock and poultry.  Emphasis will be placed on understanding depopulation strategies, food animal containment, and disposal and decontamination.  The goal of the symposium is to influence public policy with respect to mitigating the effects of a disease outbreak or other emergency event.


CEEZAD Projects:
Current Projects 2014/2015

E2E: Development of efficacious DIVA compatible vaccines for Rift Valley Fever Virus (RVFV)

PIs: Juergen Richt, Diagnostic Medicine/Pathobiology, Kansas State University;  Co-PIs:  Wenjun Ma and Bonto Faburay, Diagnostic Medicine/Pathobiology, Kansas State University;  Alan Young, Biology and Microbiology, South Dakota State University;  William Wilson, USDA Agricultural Research Service

There is an immediate and pressing need to develop effective animal vaccines to protect both wild and domestic ruminants from infection with the Rift Valley Fever Virus (RVFV).  These vaccines must be effective at inducing protective immunity, provide protection from infection in natural host species including sheep and provide the ability to differentiate vaccinated from naturally infected animals. These vaccines can then be used to protect the agricultural industry from the outbreaks of natural infection and from potential accidental or intentional exposure to this Foreign Animal Disease. Our overall goal is to develop an effective subunit vaccine which provides protection of ruminants against RVFV infection. The specific goal of this project is to express RVFV proteins in bacterial and baculovirus systems and to develop humoral and cellular immunological assays against the Rift Valley Fever Virus in natural ruminant hosts. Once developed, these tools will support of RVFV vaccine development and be directly applicable for differentiation of infected from vaccinated animals.  

Novel vaccine approaches against African Swine Fever Virus

PIs:  Yolanda Revilla Novella, ASFV Laboratory, Centro De Biologia Molecular (CBMSO), Universidad Autonoma;  Co-PIs:  Juergen Richt and Nicholas Haley, Diagnostic Medicine/Pathobiology, Kansas State University

Absence of an efficient and safe vaccine against ASFV severely hampers strategies for disease control and eradication. Thus, new strategies leading to an efficacious vaccine against ASFV are the goal of this project.  The overarching goal is to develop an ASF vaccine that offers excellent protection in swine, using a new approach to ASFV vaccinology: heterologous prime-boost vaccination, combining ASFV antigens from both DNA vaccination and recombinant ASFV proteins. The focus in the present study is to develop these protein and DNA-based vaccine components, and demonstrate their immunogenicity in pigs.  In future studies, we will test efficacy of the most immunogenic combinations, selected based on in vivo immunogenicity tests developed in the present study.  Our current proposal will be conducted jointly in the US (BRI-KSU) and Spain (CBMSO). The vaccination/challenge study in our future plans will also be done both in the US (BRI) and Spain (CBMSO).

Pathogen Detection and Discovery

PIs: W. Ian Lipkin, Center for Infection and Immunity, Columbia University; Co-PIs:  Thomas Briese, Center for Infection and Immunity, Columbia University;  Richard Hesse and Gary Anderson, Veterinary Diagnostic Laboratory, Kansas State University

Provide DHS and related agencies with state-of-the-art multiplex technologies for pathogen surveillance and discovery to protect US agricultural systems through rapid detection of newly emerging agents, and implementation of operator-safe assay platforms. 

Evaluation and Translation of Point of Need Molecular Diagnostics for Transboundary,
Emerging and Zoonotic Infectious Agents

PI:  Jessie D. Trujillo,  Center of Advanced Host Defenses, Immunobiotics and Translational Comparative Medicine, College of Veterinary Medicine, Iowa State University;  Co-PI:  Igor Morozov, CEEZAD, Kansas State University

The overall goal of this research project is the development and validation of Point of Need (PON) molecular diagnostic assays using a portable PCR device for emergency use in the field for rapid detection and differentiation of Food and Mouth Disease virus (FMDV), Rift Valley Fever virus (RVFV), and Classical Swine Fever virus (CSFV).  Additionally, this project will establish a consortium of national and international collaborators and end users to employ a five-stage evaluation/translational pipeline for the development and validation of future field deployable diagnostics devices. This pipeline is necessary of the success of this project to insure expectation of performance of emergency deployable PCR.

Field Deployable Miniature Biosensor for Genomic Signature Based ASFV and CSFV Detection and Characterization

PI:  Willy A. Valdivia, Orion Integrated Biosciences, Inc.

This proposal seeks to develop a next generation multiplexed and sensitive nucleic-acid and protein based pathogen detection and characterization platform to recognize in situ transboundary animal diseases such are ASFV and CSFV.The main characteristics of this assay will be: portability, user-friendly performance, sensitivity, specificity, low costs and integration with biosurveillance environments.

Developing a Biosurveillance and Simulation Tool Interoperability Network (BASTION) for Infectious Disease

PI:  Neil F. Abernethy, University of Washington

Develop a knowledge model representing key concepts and risk factors relevant to modeling risk of infectious disease emergence.  Develop an extensible environment to promote collaboration among modelers and scientists in CEEZAD, evaluate the ability of the system to recapture relevant risk factors in pilot risk models of major high-threat pathogens.
 

A syndromic surveillance system for early detection of emerging diseases of swine using routinely collected production, demographic and epidemiological data

PI:  Andres M. Perez, College of Veterinary Medicine, University of Minnesota;  Co-PIs:  Tim Snider, Robert Morrison, and Peter Davies, College of Veterinary Medicine, University of Minnesota  

The purpose of this project is to develop a syndromic surveillance infrastructure for early detection of emerging diseases of swine in the United States.

Deliver Web-based Continuing Education Courses for Veterinarians, Animal Health and Homeland Security Professionals

PI:  James A. Roth, Center for Food Security and Public Health, Iowa State University; Co-PI:  Claire Andreasen, College of Veterinary Medicine, Iowa State University

Increase online educational resources related to emerging, foreign, and zoonotic diseases and provide online training to graduate students, veterinarians and public health professionals to enhance awareness of these diseases. 

Completed Projects 2013/2014

Lessons Learned Information Sharing (LLIS)/ Food, Agriculture and Veterinary Defense Project

PI:  Kenneth Burton, National Agricultural Biosecurity Center (NABC), Kansas State University  

The NABC will provide continued assistance to OHA/DHS for further development of the Food, Agriculture and Veterinary Defense (FAVD) page located within the Lessons Learned Information Sharing (LLIS) website. NABC in cooperation with OHA/DHS will review and evaluate After Action Reports/Improvement Plans (AAR/IP) arising from agricultural based training exercises or actual events. Information published on the FAVD page will allow agricultural emergency managers the opportunity to review the lessons identified by NABC analysis of tabletop and field exercise AAR/IPs and then decide how those lessons might be incorporated into their own emergency response plan. A third component of NABC efforts will provide review and evaluation of AAR/IP documents looking for needs represented by common themes. These needs will be cross-referenced with existing DHS and other programs to fill training gaps within the agricultural emergency response exercises.

Completed Projects 2012/2013

Vectored equine herpesvirus type 1 (EHV-1) vaccines

PI:  Nikolaus Osterrieder, Institut fur Virolgie, Freie Universitat Berlin

The benefit of this project for DHS and the United States agriculture are generated vaccine candidates contribute to preparedness against intentional or fortuitous introduction of pathogens into poultry and livestock operations. We utilize here versatile platforms for the generation of specific vaccine candidates. We had generated infectious clones of vaccine herpesvirus strains of MDV, DEV and EHV-1. By employing a novel mutagenesis method, two-step Red recombination in Escherichia coli, we are able to insert any sequence of interest into the infectious herpesvirus clones. Making use of the established vaccine vector platforms, vaccine candidates for any given viral, bacterial or even multicellular pathogen can be produced within weeks of pathogen identification through a combination of synthetic biology (production of codon-optimized (anti)genes) and genetic engineering. As our constructs are based on modified live herpesvirus vaccines, it will guarantee authentic processing and presentation of the antigen to the immune system of chickens, ducks or horses.  For MDV and EHV-1, the vector suitability has been demonstrated earlier and the constructs were shown to be reliable vaccine candidates. Together with other projects generating and testing vaccine viruses, we provide a panel of vaccine candidates that can be tested side by side with respect to innocuousness and efficacy. The information obtained will allow a robust assessment and help in the decision-making process should vaccination, either widespread or with more limited scope (ring vaccination, temporally restricted), be deemed necessary. 

Detection and differentiation of equine alphaviruses using multiplex real-time RT-PCR assays

PI:  Udeni B.R. Balasuriya, Department of Veterinary Science, University of Kentucky;  Co-PI:  Sergey Artiushin, Department of Veterinary Science, University of Kentucky

The main objective of this proposal is to develop a panel of real-time PCR assays for the rapid detection of foreign and emerging viral and bacterial diseases of horses. Specifically we will target four foreign viral diseases (African Horse Sickness [AHSV], Equine Encephalosis [EEV], and Rift Valley Fever [RVFV] and three emerging bacterial diseases (strangles [ Streptococcus equi subsp. Equi], leptospirosis [Leptospira interrogans and other pathogenic leptospira] and contagious equine metritis [ Taylorellaequigenitalis]) in this proposed study. If real-time PCR assays are already available for any of the above agents, they will be compared for sensitivity and specificity. We will establish a network of collaborative arrangements with pre-eminent national and international laboratories, including those that are already active in this area of equine research. Once established, these protocols and reagents for the detection of foreign, emergent and zoonotic diseases of horses will be distributed to veterinary diagnostic laboratories within the United States (NAHLN).

Determine Safety and Efficacy of a Newcastle Disease Virus (NDV)-Vectored Avian Influenza Virus (AIV) in Poultry

PI:  Adolfo Garcia-Sastre, Department of Microbiology, Icahn School of Medicine at Mount Sinai; Co-PIs:  Juergen Richt and Wenjun Ma, Diagnostic Medicine/Pathobiology, Kansas State University

Generate genetically engineered Newcastle disease virus (NDV)-based vaccines expressing hemagglutinin (HA) of H5, H7 and H9 subtype avian influenza virus (AIV) in order to create an efficacious vaccine against both NDV and AIV.

Epidemiological Study of Persons with Intense Exposure to Ruminants for Rift Valley Fever Virus Infection

PI:  Gregory C. Gray, Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida

Rift Valley Fever virus (RVFV) is a zoonotic arbovirus, with human infections resulting from contact with the blood or organs of infected animals, or via bites of infected mosquitoes, ticks, or flies. Certain occupational groups such as animal workers and veterinarians are considered at higher risk of infection, and individuals with high exposure to ruminants have also been shown to have a high incidence of exposure to RVFV. Infection can cause severe disease in both animals and humans, leading to high rates of abortions (animals), disease, and death. RVFV is associated with periodic outbreaks, particularly in Africa; however, the maintenance of the virus during the inter-epidemic period (IEP), when there is low or no disease activity detected in livestock or humans, has not been fully determined. The overarching purpose of this project is to identify specific risk factors for RVFV infection in individuals with intense exposure to ruminants with the goal of providing decision makers with data to guide national public and agricultural health policy decisions.

Use of resident Canada Geese as a sentinel system to document and map avian influenza transmission within the United States

PI:  David E. Stallknecht, Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia

Develop a serology-based surveillance system that will detect local avian influenza virus transmission utilizing resident (non-migratory) Canada goose populations within the United States.

Missouri School of Journalism Doctoral Fellowship Program in Risk and Crisis Communication

PI:  Glen T Cameron, School of Journalisom, University of Missouri

Initiate a training program for graduate journalism students in communicating issues related to infectious disease and public health, particularly zoonotic and foreign animal diseases, to the public and the science journalism community

Completed Projects 2011/2012

Novel vaccine platforms: H3N2 SIV protection of swine as a proof-of-concept

PI:  Adolfo Garcia-Sastre, Department of Microbiology, Icahn School of Medicine at Mount Sinai; Juergen Richt, Diagnostic Medicine/Pathobiology, Kansas State University;  Co-PI:  Wenjun Ma, Diagnostic Medicine/Pathobiology, Kansas State University

Develop a universal vaccine platform panel for livestock starting with four widely used vectors. Specifically, 1) generate genetically engineered Newcastle Disease Virus (NDV), canarypox virus, baculovirus, and adenovirus based vaccines expressing hemagglutinin (HA) of swine influenza virus (SIV) H3N2, and 2) test these recombinant vaccines constructs for protection of pigs against SIV H3N2 as a proof of concept. 

Repository for Clinical Samples at Kansas State Veterinary Diagnostic Laboratory

PI:  Richard Hesse, Kansas State Veterinary Diagnostic Laboratory, Kansas State University;  Co-PI: Gary Anderson, Kansas State Veterinary Diagnostic Laboratory, Kansas State University

To use the Kansas State Veterinary Diagnostic Laboratory's (KSVDL) archived diagnostic samples and annual accessions to develop a repository core that can be used to help evaluate newly developed diagnostic and detections systems as well as to help determine whether a newly discovered agent was previously present in an animal population or is

Recent Publications
IIAD:

Shafagati N, Patanarut A, Luchini A, Lundberg L, Bailey C, Petricoin E 3rd, Liotta L, Narayanan A, Lepene B, Kehn-Hall K. The Use of Nanotrap Particles for Biodefense and Emerging Infectious Disease Diagnostics. Pathog Dis. 2014 Jan 21.

McReynolds SW, Sanderson MW. Feasibility of depopulation of a large feedlot during a foot-and-mouth disease outbreak. J Am Vet Med Assoc. 2014 Feb 1;244(3):291-8. 

Dohna HZ, Peck DE, Johnson BK, Reeves A, Schumaker BA. Wildlife-livestock interactions in a western rangeland setting: Quantifying disease-relevant contacts. Prev Vet Med. 2014 Mar 1;113(4):447-56. 

Morrill, JC, Laughlin RC, Lokugamage N, Wu J, Pugh R, Kanani P, Adams LG, Makino S, Peters CJ. Immunogenicity of a recombinant Rift Valley fever MP-12-NSm deletion vaccine candidate in calves. Vaccine. 2013. In Press. Accepted 1 August 2013 and epub ahead of print.

Hanna N, Ouahrani-Bettache S, Drake KL, Adams LG, Köhler S, Occhialini A. Global Rsh-dependent transcription profile of Brucella suis during stringent response unravels adaptation to nutrient starvation and cross-talk with other stress responses. BMC Genomics. 2013 Jul 8;14:459.

Shafagati N, Narayanan A, Baer A, Fite K, Pinkham C, Bailey C, Kashanchi F, Lepene B, Kehn-Hall K. The Use of NanoTrap Particles as a Sample Enrichment Method to Enhance the Detection of Rift Valley Fever Virus.PLoS Negl Trop Dis. 2013 Jul 4;7(7):e2296.

Yamazaki W, Mioulet V, Murray L, Madi M, Haga T, Misawa N, Horii Y, King DP. Development and evaluation of multiplex RT-LAMP assays for rapid and sensitive detection of foot-and-mouth disease virus. J Virol Methods. 2013 Sep;192(1-2):18-24.

Morrill JC, Laughlin RC, Lokugamage N, Pugh R, Sbrana E, Weise WJ, Adams LG, Makino S, Peters CJ. Safety and immunogenicity of recombinant Rift Valley fever MP-12 vaccine candidates in sheep. Vaccine. 2013 Jan 7;31(3):559-65.

Strelioff CC, Vijaykrishna D, Riley S, Guan Y, Peiris JS, Lloyd-Smith JO. Inferring patterns of influenza transmission in swine from multiple streams of surveillance data. Proc Biol Sci. 2013 May 8;280(1762):20130872.

Mardones FO, Zu Donha H, Thunes C, Velez V, Carpenter TE. The value of animal movement tracing: a case study simulating the spread and control of foot-and-mouth disease in California. Prev Vet Med. 2013 Jun 1;110(2):133-8.

Gomez G, Adams LG, Rice-Ficht A, Ficht TA. Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Front Cell Infect Microbiol. 2013 May 16;3:17.

Gomez G, Pei J, Mwangi W, Adams LG, Rice-Ficht A, Ficht TA. Immunogenic and invasive properties of Brucella melitensis 16M outer membrane protein vaccine candidates identified via a reverse vaccinology approach. PLoS One. 2013;8(3):e59751.

Student Opportunities at ZADD

To learn about student opportunities at the Institute for Infectious Animal Diseases (IIAD) please visit http://iiad.tamu.edu/educational-programs/student-opportunities/

Technology Transition

E2E project information may be found at this link

Research Partners

For a list of Institute for Infectious Animal Diseases (IIAD) partners and stakeholders please click here.

For a list of Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) partners and stakeholders please click here.

ZADD Resources

To learn more about Institute for Infectious Diseases (IIAD) resources please visit http://iiad.tamu.edu/resources/.

To learn more about the Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) visit http://www.ceezad.org/resources/.